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    Please use this identifier to cite or link to this item: http://ir.nknu.edu.tw/ir/handle/987654321/15920


    題名: 三種茄科植物辣椒、蕃茄、茄子組織培養系統之建立與比較
    The Establishment and Comparison of Tissue Culture System of Three Solanaceous Plants(Capsicum annuum L.、Lycopersicon esculentum Mill.、Solanum melongena L.)
    Authors: 戴守音
    Shou-Yin Tai
    貢獻者: 廖麗貞
    Li-Jen Liao
    Keywords: 辣椒;蕃茄;茄子;組織培養
    Date: 2004-08-30
    Issue Date: 2011-09-27 16:21:47 (UTC+8)
    Abstract: 本研究以辣椒、蕃茄、茄子等三種茄科植物無菌苗的莖作為培植體,以測試不同植物生長調節劑的組合,對誘導癒合組織及芽再生的影響。實驗結果如下:
    1.以MS培養基添加 10 ppm NAA與10 ppm NAA + 0.1 ppm kinetin的組合,可誘導產生最大量的辣椒癒合組織,經過14天的培養後,其生成率分別為72 %與47 %。
    2.以MS培養基添加1 ppm NAA+ 1 ppm kinetin誘導蕃茄癒合組織,14天後其增生率達65.8 %。將略帶綠點的癒合組織移植到添加1.69~2.82 ppm BA的MS培養基,可產生綠色芽點。再繼續培養至四十二天時,芽點逐漸長成不定芽,再以MS培養基添加花寶四號0.01 %~0.05 %誘導發根,成為再生新植株。
    3.以MS培養基添加0.1 ppm NAA誘導茄子生成癒合組織,14天後其增生率達81 %。而0.93 ppm NAA能誘導癒合組織出現芽點,長出不定芽。以MS培養基添加花寶四號0.01 %~0.05 %可誘導不定芽發根,生成再生植株。
    本研究結果可由辣椒、蕃茄和茄子莖節間誘導產生癒合組織,並且能順利由蕃茄和茄子的癒合組織,進一步誘導出再生植株,但在辣椒的植株再生方面仍有待努力。
    This study was to investigate the callus induction and bud regeneration of three solanaceous plants by using stem segments. To reach the purpose of this study, we composited MS medium with various concentrations of plant growth regulators to determine the efficiency of callus induction and bud regeneration. The results as summarized as follows:
    1. Callus formation in Capsicum annuum L. can be induced by MS medium supplemented with 10 ppm NAA. The callus proliferation can get in 72% after 14 days.
    2. Callus formation in Lycopersicon esculentum Mill. can be induced by MS medium supplemented with 1ppm NAA+ 1 ppm kinetin. Than the callus transplanted to MS medium supplemented with 1.69~2.82 ppm BA could induce shoot formation. It is most helpful to induce root formation when MS medium was supplemented with Hyponex No.4 0.01%-0.05%.
    3. Callus formation in Solanum melongena L. can be induced by MS medium supplemented with 0.1ppm NAA. Than the callus transplanted to MS medium supplemented with 0.93 ppm NAA could induce shoot formation. It is most helpful to induce root formation when MS medium was supplemented with Hyponex No.4 0.01%-0.05%.
    In this study, the plantlets regeneration can be induced in Lycopersicon esculentum Mill. and Solanum melongena L.. But the Capsicum annuum L. could only develop to the callus formation without bud regeneration, so it need to work hard in the aspects of their tissue culture.
    Appears in Collections:[生物科技學系] 博碩士論文
    [生科系] 廖麗貞

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