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    高師機構典藏 NKNUIR > 理學院 > 化學系 > 期刊論文 >  Item 987654321/23656
    Please use this identifier to cite or link to this item: http://ir.nknu.edu.tw/ir/handle/987654321/23656


    題名: Extraction of Substance P by Functionalized Iron Oxide Nanoparticles for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis
    Authors: 張玉珍
    Yen-Hsiang Chang;Zhi-Meng Chen;Hsien-Yu Peng;Yuan-Jen Tsai;Tzer-Bin Lin;Gin-Den Chen;C. R. Chris Wang;Sarah Y. Change
    Keywords: Substance P;Rat spinal cord samples;Iron oxide nanoparticles;MALDI/MS
    Date: 2011-08
    Issue Date: 2015-04-27 11:34:45 (UTC+8)
    Abstract: Functionalized iron oxide nanoparticles (IONPs) were used as concentrating probes to extract substance P (SP) from aqueous solution before matrix-assisted laser desorption/ionization mass spectrometric (MALDI/MS) analysis. Adsorption of SP onto the nanoparticles was mainly due to electrostatic interaction. After simple washing, the SP-adsorbed nanoparticles were directly analyzed by MALDI/MS. With this concentration strategy, the limit of detection (LOD) of SP in 500 μL buffer solution (pH 8) was 0.1 nM. In addition, the nanoparticles could isolate SP from a solution with high contents of salts and surfactants, thus alleviating suppression effect during MALDI/MS analysis. This new method was successfully applied to the determination of SP in rat spinal cord samples. The LOD and recovery of SP in a rat spinal cord sample were 3.1 nM and 85.7%, respectively.
    關聯: Journal of the Chinese Chemical Society / Volume 58, Issue 4, pages 457–462
    Appears in Collections:[化學系] 期刊論文
    [化學系] 張玉珍

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