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    題名: 奇異花蝴蝶蘭雜交育種及其實生苗繁殖系統之建立
    Studies on the establishment of the hybridization and seedling propagation system in novel Phalaenopsis
    Authors: 蔡美源
    Mei-Yuan Tsai
    貢獻者: 廖麗貞
    Li-Jen Liao
    Keywords: 蝴蝶蘭;實生苗;奇異花
    Phalaenopsis;seedling;novel
    Date: 2003-08-22
    Issue Date: 2011-09-27 16:30:45 (UTC+8)
    Abstract: 本研究主要在探討不同奇異花蝴蝶蘭雜交品種組合的著果率,授粉後蒴果培養天數和溫度與雜交胚萌芽之關係,並建立實生苗大量繁殖模式。
    在試驗品種裡,紫紅色、粉紅色系,最易授粉;Dtps. Minho Princess. “三傑”、朱紅色、黃綠色系,不易授粉。
    在夏季溫度(35/25℃)環境下授粉者,不但果莢小,而且果莢未成熟即爆裂,有些甚至十天就夭折,果莢變黃而脫落。
    授粉後98天所採收的果莢進行播種,已具有發芽能力;然而,仍以在授粉後126天左右之成熟果莢有最佳之培養效果。
    對於蝴蝶蘭未成熟胚之發育的觀察發現,播種兩星期後,未成熟胚吸水膨大並撐破種皮,形成深黃綠色之原球體,將其移到增殖用的B培養基的中母瓶,即為第二繼代;當第二繼代之幼胚長出1公分時,把它們移到幼苗成長用的P培養基的子瓶,即為第三繼代。
    觀察並記錄芽體的鮮重與增殖速率,當每單株鮮重達0.3克,葉片長達3-5公分時,即移到瓶外栽種。
    目前建立之蝴蝶蘭實生苗的培育系統,從播種到出瓶最快需時約8個月,比一般文獻記載縮短2-4個。
    This study is aimed at the ratio of fruit setting, initial culture stage and growth temperature between different hybridized combinations of Phalaenopsis, and also establishing the hybridization and seedling propagation system in novel Phalaenopsis.
    In all species studied, the purple-red and pink species are easier to pollinate than the Dtps. Minho Princess, dark red and yellow-green species.
    The capsules of the Phalaenopsis which pollinated in summer are smaller and the immature capsules fell off easily. Some capsules even died in ten days and the capsules became yellow and fell off.
    The capsules grew 98 days after pollination have the ability to germinate. However, the capsules which grew 126 days after pollination have the best response in culture.
    We observed the growth of immature embryos and found that the embryos absorbed water, swelled and then the seed coats was beginning to break and became dark yellow-green protocorm after two weeks. Then we transferred them to B medium for proliferation as the second subcultures. When the seedling length of second subculture were 1 cm in length, we transferred them to P medium for seedling growth as the third subcultures.
    When the fresh weight of plantlets arrived about 0.3 g with the leaves 3-5 cm in length, it would be transferred and culture outside the bottle.
    The hybridization and seedling propagation system in novel Phalaenopsis is established at least about eight months from seeding to transferring outside the bottle. It is 2-4 months faster than the previous reports.
    Appears in Collections:[生物科技學系] 博碩士論文
    [生科系] 廖麗貞

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