在試驗品種裡，紫紅色、粉紅色系，最易授粉；Dtps. Minho Princess. “三傑”、朱紅色、黃綠色系，不易授粉。
目前建立之蝴蝶蘭實生苗的培育系統，從播種到出瓶最快需時約8個月，比一般文獻記載縮短2-4個。 This study is aimed at the ratio of fruit setting, initial culture stage and growth temperature between different hybridized combinations of Phalaenopsis, and also establishing the hybridization and seedling propagation system in novel Phalaenopsis.
In all species studied, the purple-red and pink species are easier to pollinate than the Dtps. Minho Princess, dark red and yellow-green species.
The capsules of the Phalaenopsis which pollinated in summer are smaller and the immature capsules fell off easily. Some capsules even died in ten days and the capsules became yellow and fell off.
The capsules grew 98 days after pollination have the ability to germinate. However, the capsules which grew 126 days after pollination have the best response in culture.
We observed the growth of immature embryos and found that the embryos absorbed water, swelled and then the seed coats was beginning to break and became dark yellow-green protocorm after two weeks. Then we transferred them to B medium for proliferation as the second subcultures. When the seedling length of second subculture were 1 cm in length, we transferred them to P medium for seedling growth as the third subcultures.
When the fresh weight of plantlets arrived about 0.3 g with the leaves 3-5 cm in length, it would be transferred and culture outside the bottle.
The hybridization and seedling propagation system in novel Phalaenopsis is established at least about eight months from seeding to transferring outside the bottle. It is 2-4 months faster than the previous reports.